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EVlution™

Tangential Flow Filtration of EVs Made Easy

EVlution™ TFF brings tangential flow filtration to your bench - scalable EV isolation with a small footprint, disposable sample-contacting parts, and a concentration step that delivers a small, assay-ready final volume.

EVlution TFF

EVlution™ is a compact, affordable benchtop tangential flow filtration (TFF) system engineered for high-yield concentration and purification of extracellular vesicles (EVs) and other biomolecules. Process up to 1 litre of sample in just 90 minutes, with Switch Flow™ technology reducing retentate volumes to less than 10 mL - maximising recovery while minimising loss. Compatible with 12 polyethersulfone hollow fibre cartridge pore sizes (1 kDa–1,000 kDa), EVlution™ delivers the flexibility, purity, and throughput that ultracentrifugation can't match, at a fraction of the cost.

Simple Principles and Workflow

TFF provides an effective method of size-based separation of biomolecules, including EVs. The characteristics of TFF design makes it an excellent choice for workflows at the lab scale. The adaptable design of EVlution™ supports a wide range of hollow fiber filter configurations, making it a flexible solution for diverse laboratory-scale applications. With an intuitive interface and streamlined operation, EVlution™ simplifies complex filtration processes while delivering reliable performance.

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Dead-end filtration passes the entire sample volume through the filter, leading to filter clogging and a low permeate rate. TFF recirculates samples across the filter surface, sweeping the filter surface to prevent clogging.

 

The workflow for using EVlution™ TFF allows processing of a 1 litre sample in 90 minutes.

 

Sample flow paths in EVlution™ TFF. The blue path represents the flow of the sample entering through the inlet, across the TFF cartridge and looping back as retentate. The green path represents the portion of the sample that passes through the membrane and exits separately as permeate.

EVlution™ works with a range of hollow fibre filters with different pore sizes for different applications, which include:

  • Recovering proteins, antibodies, and EVs from cell culture media and other starting materials.
  • Concentrating and purifying proteins, peptides, and nucleic acids.
  • Depletion of FBS-associated EVs 
  • Buffer exchange (diafiltration)

Features and Benefits

Compared to ultracentrifugation, TFF is a more efficient method for processing large sample volumes. Using the 260 cm2 filter, EVlution™ allows researchers to concentrate and purify EVs from up to 3 litres of starting material.

Key Features and Benefits of the EVlution™ system

Switch Flow™ for low retentate volume

Entirely disposable sample contact surfaces 

 Affordable

 12 pore sizes available 

 Easy to use. Minimal hands-on time 

 

EVlution™ has been validated in EV workflows 

 

Comparison of Common EV Concentration Methods
 

Ultra-

centrifugation

Precipitation

EVlution TFF

Price

 High Low Moderate
Purity Moderate Low

High

Throughput

 Low High High

Yield

 Moderate Moderate High