Introduction to amplification-free gene editing
characterization using HiFi sequencing
Detect and accurately measure the efficiency of both on- and off-target gene editing
Gene editing approaches have become an important tool in research and the development of potential novel therapeutics.
CRISPR-Cas9, base editing, prime editing, transposons and more have great potential to alter genomes in a controlled way. However, each of these methods can have inaccuracies that may pose potential safety risks if introduced in humans. Some of these unintended outcomes include larger structural variants, and insertions or deletions, or concatemers of inserts, that could be missed by using short-read sequencing methods.
HiFi sequencing allows you to confidently assess those larger variants as well as single base changes to determine
- On- and off-target editing effects
- Insertional mutagenesis
- The potential safety of resulting constructs
Highly accurate HiFi reads for gene editing research - App Note(external link)
With highly accurate long reads (HiFi reads), powered by Single Molecule, Real-Time (SMRT) sequencing technology, you can more comprehensively validate gene editing techniques such as CRISPR-Cas9 approaches.
Use a variety of approaches such as amplicon-based sequencing, amplification-free targeted sequencing, and whole genome sequencing to quantify genome editing outcomes, assess insertion sites and understand the effects of haplotypes and SNVs on genome editing.
